Akademik Veri Yönetim Sistemi
Dirican O., Buluş A. D., Oğuztüzün S.(Yürütücü)
Yükseköğretim Kurumları Destekli Proje, 2022 - 2023
Background: The data,
which we have revealed as an original scientific study, were made on
individuals who were diagnosed with "obesity" in the pediatric clinic
regarding the emergence of obesity in childhood, which is one of the most
common health problems of today.
Aim: Presence of
deletions in the GST-M1 and GST-T1 gene regions that encode protein expressions
of isozymes of the Glutathione S-Transferase enzyme, which is known to have an
important role in the intended detoxification mechanism.
Metod: In our
study, we started to work with 110 childhood individuals diagnosed with
obesity. However, since the efficiency of the molecular material obtained from
these individuals (DNA levels in terms of purity and concentration) did not
allow for further analysis due to their inability to work in the next step or
the inability to obtain results, these individuals were technically excluded
from the study. As a result of the study, the results of 60 individuals
diagnosed with obesity from all three gene regions were obtained. The results
of this analysis are also statistically discussed along with some clinical and
demographic information. In our study, some of the GST-M1 and GST-T1 deletion studies
were performed by qPCR melting-curve analysis, and p53 single nucleotide
polymorphism analysis was performed by sequencing (sequence) method.
Results: Some
clinical data of the patients such as Glucose, Urea, Creatine, Total Protein,
Albumin, Cholesterol, Triglyceride, HDL, LDL, ALT, AST, GGT, T4, TSH, Cortisol,
Insulin, Vitamin D, ACTH, WBC, HGB, Hepatobiliary USG, In addition to the
genetic data obtained, some demographic data (age, gender, etc.) are provided.
In the light
of these data, when obesity is considered epidemiologically, the polymorphic
state seen after mutations in these gene regions and the hereditary state of
childhood obesity have been tried to be revealed. We believe that the results
we have obtained will shed light on other studies to be conducted in the same
field. In addition, we think that other studies to be conducted in other
geographical regions, different socio-economic groups and similar age groups
will contribute to a better understanding of childhood obesity in terms of molecular
metabolism.