Akademik Veri Yönetim Sistemi
Tez Türü: Yüksek Lisans
Tezin Yürütüldüğü Kurum: Baqai Medical University, Health and Sciences, ANATOMY, Pakistan
Tez Danışmanı: Prof. Dr. Kishwar Sultana
Tezin Onay Tarihi: 2014
Tezin Dili: İngilizce
Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
Desteklendiği Program: Diğer
Özet:
Fluconazole is a subclass of triazole antifungal agents, is available as oral tablets, powder or for oral
suspension, and as a sterile solution for intravenous administration.
Fluconazole is designated chemically as 2,4-difluoro-α,α1-bis benzyl alcohol in the form of white crystalline
solid which is slightly soluble in water and saline.
Fluconazole oral suspension contains 350 mg or
1400 mg contains sucrose, citric acid anhydrous, sodium benzoate, sodium
citrate, colloidal, silicon dioxide, titanium dioxide, xanthan gum, and natural
orange flavor. After reconstitution with 24 ml of distilled water or purified water
each ml of reconstituted suspension contains 10 mg or 40 mg of fluconazole.
Animals
are divided into 4 groups. The rats of group A are taken as control group. In
group B rats are kept nothing per mouth at 12 hours prior to induction of
septic shock produce by Candida albican. Group C animals are administrated with
fluconazole at doses of 3mg/kg, 10mg/kg and 30mg/kg after the septic shock
produce by candida albican. Since fluconazole at a higher dose has clearly
shown to decrease tissue injury. We have decided to keep fluconazole dose as
constant and vitamin E would be administered in dose of 10mg/100gm of body
weight in group
D. Both the drugs will be administered after inducing septic shock. After the
study, animals will be sacrificed and lungs will be obtained within 8 hours of
septic shock.
Lungs will be taken out of sacrificed
animals and will be fixed with paraffin and then sectioned and stained by eosin
and haemotoxylin. With the help of cardiac puncture blood samples were taken to
estimate the infectious markers. Study of CBC and CRP was done. The levels were
estimated and compared with control and correlated with histologic findings.
The lung histology will be determined by mucosal thickening and inflammatory
cells.
All
these results were in favor of group D fluconazole along with vitamin E that
shows significant results in all parameters. There was significant correlation
between control and fluconazole along with vitamin E treated group because
vitamin E considerably reduced the histopathological changes in lungs
parenchyma.
It
was concluded from this study that the antioxidant property of vitamin E
decreases the lung tissue injury and oxidative stress when given along with fluconazole.