Investigation of the Effects of Extracts of Ornithogalum sigmoideum Freyn & Sint. and Smilax excelsa L. on GST Isozymes in the HepG2 Cell Line.
Türk Doğa ve Fen Dergisi, cilt.15, sa.3, ss.154-162, 2026 (TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 15 Sayı: 3
- Basım Tarihi: 2026
- Doi Numarası: 10.46810/tdfd.1984984
- Dergi Adı: Türk Doğa ve Fen Dergisi
- Derginin Tarandığı İndeksler: TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.154-162
- İstanbul Gelişim Üniversitesi Adresli: Evet
Özet
This study aimed to investigate the effects of extracts obtained from the aerial and underground
parts of Ornithogalum sigmoideum Freyn & Sint. (Sakarca, Çökülce, Tükürük otu, and Karga
sarımsağı) and Smilax excelsa L. (melocan, dikenucu, kırçan, melevcan, merülcen, saparna)
plants on GST (Glutathione-S-Transferase) isozymes in the HepG2 (Hepatocellular Carcinoma
Cell Line) liver cancer cell line. Plant samples were collected from the Perşembe district market
in Ordu province in May, which is the harvesting and consumption season for the plants, and
their extracts were prepared. Liver cancer cells were cultured in a specially prepared medium
using standard cell culture methods. Plant extracts were applied to the cell lines at various
concentrations, and cell viability was examined at 24, 48, and 72-hour intervals. The viability of
liver cancer cells was investigated using the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl
tetrazolium bromide) test method. Based on the determined IC50 values (expressed in
micrograms per milliliter, µg/mL), the concentration ranges required to maintain 50% viability
of the plant extracts in the HepG2 cell line were determined. GST enzyme activity assays were
performed to determine the total GST-specific activity following plant extract treatments, and
results were evaluated using appropriate statistical analyses (e.g., One-Way ANOVA followed
by Tukey's post-hoc test, p < 0.05). Immunocytochemical staining demonstrated the presence of
GST enzymes involved in the apoptotic pathway in the HepG2 cell line at the microscopic level.
In conclusion, it was determined that each plant extract affected the expression of GST enzymes
in the liver cell line differently (suppression or increase), suggesting that these extracts warrant
further detailed pharmacological investigations.