Immunohistochemical investigation of GST-M, GST-P, and TP53 gene expression in obesity patients under laparoscopic sleeve gastrectomy

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Dirican O., Kaygın P., Kaplan B., Yılmaz Sarıaltın S., Güler Şimşek G., Oğuztüzün S., ...Daha Fazla

TISSUE AND CELL, cilt.98, sa.1, ss.1-7, 2025 (SCI-Expanded, Scopus)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 98 Sayı: 1
  • Basım Tarihi: 2025
  • Doi Numarası: 10.1016/j.tice.2025.103143
  • Dergi Adı: TISSUE AND CELL
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Veterinary Science Database
  • Sayfa Sayıları: ss.1-7
  • İstanbul Gelişim Üniversitesi Adresli: Evet

Özet

Obesity is linked to increased oxidative stress, contributing to various comorbidities. Glutathione S-transferases (GSTs) enzymes and tumor suppressor protein 53 (TP53) are key regulators of oxidative stress. This study aimed to examine the expression of GST-M, GST-P, and TP53 in sleeve gastrectomy tissues of obese patients. In this retrospective study, 126 obese patients who underwent sleeve gastrectomy were analyzed. Immunohistochemical staining assessed the expression of GST-M, GST-P, and TP53 in sleeve gastrectomy tissue samples. Correlation between protein expression and association with demographic, clinical, and serological data was examined. GSTP and GST-M exhibited low expression (absent/weak), while TP53 showed significantly higher expression in the sleeve gastrectomy tissue of patients with obesity. A positive and significant correlation was identified between GST-M and TP53 staining levels, while GST-P expression level did not display any correlation with GST-M and TP53. Distinct patterns of protein expression for GST-P, GST-M, and TP53 in the sleeve gastrectomy tissues of obesity patients. TP53 plays a key role in oxidative stress regulation in obesity, whereas GST-M and GST-P appear less effective.