Veterinarski Arhiv, cilt.72, sa.5, ss.235-248, 2002 (Scopus)
In this study, the cellular localisation and distribution of BVD viral antigens in genital system tissues were investigated in 65 non-pregnant dairy cows and in 65 bulls. For this study genital system tissue samples, taken from slaughtered bulls with no pathological lesions and from females having reproductive problems, were marked using the immunoperoxidase method on their paraffin sections. BVD viral antigens were determined in 15 of the 65 non-pregnant dairy cows (consistent with cell culture results) using the indirect immunoperoxidase method. BVD viral antigens were present in macrophage-like cells in the stroma of the ovaries and uterus. No BVD viral antigens were observed in the samples of testicles, epididymis, vesicula seminalis, or prostate in the male animals. In all animals, including BVDV-positive ones, no pathologic lesions were observed, except periodically non-specific subepithelial or stromal mononuclear cell infiltrations. Most of these cells were seen to be formed by lymphocytes and macrophages.