© 2021. Sinem Gürkan Aydin Arzu Özgen; published by UIKTEN. This work is licensed under the Creative Commons Attribution‐NonCommercial‐ NoDerivs 4.0 License.Biofuels are fuels of biological origin, and they are among the prominent energy sources from renewable energy sources in recent years. Unlike fuels such as petroleum derivatives, coal, natural gas, biofuels are renewable and sustainable energy sources. Biofuels can be obtained by thermochemical or biochemical methods from agricultural and forestry products, animal and vegetable residues and wastes, organic origin domestic, industrial and urban wastes. Global warming caused by greenhouse gas effect is today shown as the most important common environmental problem in the world. The most important advantage of using biofuels in environmental terms is that there will be a reduction of greenhouse gas impact and acid rain. In this study, it is aimed to produce and characterize jet fuel by converting from pumpkin (Cucurbita pepo) seed oil, which is a sustainable bio energy source, into biofuel, by transesterification method. It has an important place in matters such as environment, sustainability, and reduction of carbon emissions. Biojet fuel produced using pumpkin seed oil, which is a sustainable resource, is a candidate product for development and commercialization.