Potential antiinflammatory effect of fungal polysaccharide extract from leucoagaricus leucothites via activating p38/erk phosphorylation in bv-2 cells

DUMLUPINAR B., Karatoprak G. Ş.

Latin American Journal of Pharmacy, vol.37, no.5, pp.880-887, 2018 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 37 Issue: 5
  • Publication Date: 2018
  • Journal Name: Latin American Journal of Pharmacy
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.880-887
  • Keywords: Anti-inflammatory activity, COX-2, Cytokines, INOS, MAPK
  • Istanbul Gelisim University Affiliated: No

Abstract

© 2018, Colegio de Farmaceuticos de la Provincia de Buenos Aires. All rights reserved.This study aimed to investigate some biological activities and antiinflammatory potential of polysaccharide isolated from Leucoagaricus leucothites in LPS-activated BV-2 cells. Here, we investigated the antioxidant and antimicrobial potential of polysaccharide extract of L. leucothites (LLP) and the effect of LLP on lipopolysaccharide (LPS)-induced inflammatory response and the underlying molecular mechanisms in BV-2 cells. Chemical analysis of LLP were determined by high-performance liquid chromatography (HPLC). Cytotoxicity and productions of TNF-α and IL-6 were determined by ELISA. In addition, intracellular mitogen-activated protein kinase (MAPK) signaling pathway was analyzed by Western blot and cyclooxygenase-2 (COX-2) and nitric oxide synthase (iNOS) expression were measured by real time-PCR. LLP inhibited LPS-stimulated IL-6 and TNF-α. In addition, the inhibitory action of LLP not only suppressed COX-2 and iNOS, but also inhibited the activation of p38/ERK MAPKs. Our data suggested that LLP exhibit anti-inflammatory activity that might contribute to the prevention of chronic inflammatory diseases.