The axon number of the rat sciatic nerve: A stereological study Sıçan siyatik sinir akson sayısı: Stereolojik bir çalışma


Ragbetli C., Yalama M., Kati I., TUNALI M. B., ESENER O. B. B., Bozkurt H. H., ...More

Kafkas Universitesi Veteriner Fakultesi Dergisi, vol.19, no.4, pp.635-639, 2013 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 19 Issue: 4
  • Publication Date: 2013
  • Doi Number: 10.9775/kvfd.2013.8587
  • Journal Name: Kafkas Universitesi Veteriner Fakultesi Dergisi
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.635-639
  • Keywords: Axons number, Sterology, Rat, Sciatic nerve
  • Istanbul Gelisim University Affiliated: No

Abstract

This study was designed to determine with stereological methods the number of axons in the sciatic nerve as a result of peripheral nerve blockage following injection of lidocaine and prilocaine into hind limb muscle. Nine adult female Sprague-Dawley rats weighted 150-200 g were used in the study. Ketamine was employed intraperitoneal to the rats as 50 mg/kg. The prilocaine and lidocaine were equally (5 mg/ml) injected to the left and right limbs of the 4 rats. As a control group, isotonic sodium chloride (0.9%) was performed as 0.2 ml into the extremities of the remaining 5 rats. When the application completed, surrounding muscles and soft tissues as well as sciatic nerve were dissected as 0.5×1.0 cm length. In order to determine neurotoxic effects of lidocaine and prilocaine on sciatic nerve, the number of axons was computed by unbiased stereological method. Following of the square root transformation, Mann-Whitney U test was performed to compare groups. When the unbiased comparisons were executed to both groups, the effects of lidocaine and prilocaine on sciatic nerve were found statistically non-significant.