Akademik Veri Yönetim Sistemi
Central European Journal of Immunology, cilt.46, sa.4, ss.474-480, 2021 (SCI-Expanded)
© 2021 Termedia Publishing House Ltd.. All rights reserved.Introduction: Gingival crevicular fluid (GCF) is an important resource for detecting inflammatory biomarkers related to periodontal disease. The purpose of this research was to identify the possible relation between cytokine levels and periodontal status. Material and methods: GCF samples collected from 25 periodontally healthy individuals, 24 with gingivitis, and 24 with periodontitis were analyzed using the MAGPIX system with a Bio-Plex Pro Human Cytokine 27-plex kit. Gingival index (GI), periodontal pocket depth (PPD), plaque index (PI), and clinical attachment level (CAL) were measured from each tooth to determine each patient's periodontal status. Results: All clinical parameters showed statistically significant differences between groups. While interleukin (IL)-1β, IL-6, macrophage chemotactic protein 1 (MCP-1), interferon g-induced protein 10 (IP-10) and vascular endothelial growth factor (VEGF) levels were statistically significantly higher in patients with periodontitis compared to periodontally healthy subjects, IL-15 levels were found to be statistically significantly higher in periodontally healthy individuals compared to periodontitis (p < 0.05). Also, IL-1β and IP-10 showed positive correlations with PPD, CAL, GI and bleeding on probing (BOP). Conclusions: It is considered that IL-1β, IL-6, IL-15, MCP-1, VEGF and IP-10 could be related to periodontal disease and health and can be used as an adjunct to clinical examination in future research design. Scanning cytokines in GCF with a multiplex immunoassay technique is consequential, especially because many cytokine types are found in one sample.