DERİ KANSERİNDE GST İZOZİMLERİNİN EKSPRESYONLARININ ROLÜ


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Dirican O.

9th INTERNATIONAL ERCIYES CONFERENCE ON SCIENTIFIC RESEARCH, Kayseri, Turkey, 17 - 18 July 2023, pp.52-63

  • Publication Type: Conference Paper / Full Text
  • City: Kayseri
  • Country: Turkey
  • Page Numbers: pp.52-63
  • Istanbul Gelisim University Affiliated: Yes

Abstract

Skin Cancer is triggered by oxidative stress caused by free radicals, which are formed by the interaction of various genetic and environmental factors (such as exposure to ultraviolet (UV) rays, smoking, genetic predisposition and immune system problems). Glutathione Stransferases (GSTs) are a family of detoxifying enzymes that protect the cell against chemical carcinogens, toxic chemicals and reactive oxygen species. GST enzymes help reduce DNA damage by neutralizing harmful compounds caused by free radicals. Thus, adequate levels of GST enzymes play an important role in preventing skin cancer and protecting cells from damage. GST pi (GSTP) is an important member of this enzyme system. In this study, we aimed to examine the expression of GSTP isozyme in 62 skin tumors and normal tissues. GSTP expression levels of tumor and normal tissues were evaluated by immunohistochemical method and compared with Mann Whitney-U test. Relationships between clinical and demographic data and expression levels were evaluated with Mann Whitney-U and Kruskal Wallis tests. Correlation analyzes were performed using the Spearman rank correlation test. Although GSTP expression was higher in tumor tissues than in normal tissues, no significant difference was found between groups in terms of GSTP expression (p>0.05). A statistically significant and positive correlation was found between GSTP expressions of tumor and normal tissues (p<0.001). A positive and significant correlation was also observed between the age of the patients and the tumor size (p=0.040; p<0.05). It was observed that the expression level in normal and tumor tissues from male patients was higher than in female patients. Moderately differentiated tumors showed higher GSTP expression than well-differentiated tumors. It was observed that the mean GSTP expression of squamous cell carcinoma tissues was higher than basal cell carcinomas. When evaluated according to tumor stage and size, the highest expression was found in tissues with T2 stage and 2-4 cm in size. In conclusion, the high expression of GSTP in skin tumor tissues indicates that these parameters may have an important role in the pathogenesis and progression of the disease. The effectiveness of GST enzymes in both cancer prevention and treatment helps protect cells by combating oxidative stress.