Expressions of GSTO1, GSTP1, GSTM1, GSTS1 isoenzymes in NSCLC tissues


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Dirican O.

3rd International Multidisciplinary Cancer Research Congress, İstanbul, Turkey, 7 - 10 September 2023, pp.83

  • Publication Type: Conference Paper / Summary Text
  • City: İstanbul
  • Country: Turkey
  • Page Numbers: pp.83
  • Istanbul Gelisim University Affiliated: Yes

Abstract

Introduction and Aim: Glutathione S-Transferases (GSTs) are Phase II enzymes which are involved in metabolism of various xenobiotics including the drugs. GSTP1 and GSTM1 isoenzymes have the role of protecting the lung tissue by catalyzing the conjugation of carcinogens with glutathione. In this study, we investigated the immunohistochemical staining characteristics of GSTOmega (GSTO1), GSTpi (GSTP1), GSTmu (GSTM1), p38, bcl-2 and caspase-3 in adenocarcinoma (n=20) and squamous cell carcinoma (n=20) lung tumor tissues from 40 patients.

Materials and Methods: Non-small cell lung cancer (NSCLC) tissues of patients were compared according to their immunohistochemical staining intensity from the patients. Relationships between GSTO1, GSTP1, GSTM1, p38, bcl-2 and caspase-3 expressions in carcinoma tissue were examined by the Mann Whitney-U test, and the clinicopathological data were examined by the Spearman correlation rank test.

Results: The results showed that GSTO1, GSTP1, GSTM1, p38, bcl-2 and caspase-3 expressions were significantly higher in lung tumor group than benign lung group (p<0.01). However, no statistically significant differences in the level of GSTSigma1 protein expression between tumor and benign lung groups (p>0.05). p38, caspase-3, bcl-2 and GSTSigma expressions were positively correlated in the tumor group (p<0.01). The higher expressions of GSTP1, GSTM1 and caspase-3, p38 in tumor group could be important in lung cancer progression and development. Conclusions: As a result, the difference of the GSTO1, GSTP1, GSTM1 isoenzymes expressions between the groups show that they play an important role in the diagnosis of NSCL carcinoma.