Objective: Aim of this study is to demonstrate the reliability and validity of Adverse Childhood Experience (ACE) Questionnaire. Methods: This research was carried out with 50 patients who consulted in Davranis Bilimleri Ensti-tüsü. The participants were aged between 18 and 65 years with no current alcohol and substance abuse, no mental retardation, no organic mental disorder. Participants were assessed with the socio-demographic data form, the Adverse Childhood Experience Turkish Form (ACE-TR) and the Symptom Assessment-45 Questionnaire (SA-45). On the statistical analysis, item-total score correlation coefficients, internal consistency coefficient and correlation values with other scales were calculated. Results: The mean age of the study population was 33.77±13.24 years and 48% (n=24) of the sample group were female and 52% (n=26) were males. The Cronbach’s alpha value of the total score of the scale was 0.742, which indicates that it has a consistency reliability of 74%. The single component solution was obtained as the result of the principal components analysis. Correlations between ACE-TR and SA-45 sub-components were statistically significant. Conclusion: Results demonstrated that the Adverse Childhood Experience Turkish Form (ACE-TR) was a valid and reliable instrument, which may serve as a screening tool for childhood trauma in large populations.