Cytotoxicity analysis of strontium ranelate on cultured human periodontal ligament fibroblasts: A preliminary report

ER K., Polat Z. A., Özan F., TAŞDEMİR T., Sezer U., Siso Ş. H.

Journal of the Formosan Medical Association, vol.107, no.8, pp.609-615, 2008 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 107 Issue: 8
  • Publication Date: 2008
  • Doi Number: 10.1016/s0929-6646(08)60178-3
  • Journal Name: Journal of the Formosan Medical Association
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.609-615
  • Keywords: Cell culture, Cytotoxicity, Resorption, Strontium ranelate, Trauma
  • Istanbul Gelisim University Affiliated: No

Abstract

Background/Purpose: The aim of this study was to analyze the cytotoxicity of strontium ranelate (SR) on human periodontal ligament fibroblasts (PDL cells) in vitro. Methods: PDL cells were obtained from healthy human third molars and cultured in Dulbecco's Modified Eagle's Medium. The experimental groups were: G1, cultures treated with fresh medium (control); and G2, G3, G4 and G5: treated with SR at 20, 10, 5 and 2.5 mg/mL, respectively. The experimental times were 1, 6, 12 and 24 hours (short-term) for viability, and 2, 4, 6 and 8 days (long-term) for cell survival. The cells were counted using a hemocytometer. Data were then analyzed by one-way ANOVA and Tukey's tests (p < 0.05). Results: Cultures treated with the highest SR concentrations (G2 and G3) had significantly lower cell viability and cell numbers (p < 0.05) than those in G1, G4 and G5. SR at 2.5 mg/mL was non-cytotoxic to PDL cells. Conclusion: SR was non-toxic at appropriate concentrations. Preclinical tests are needed to further assess its safety and effectiveness for tooth resorption prior to clinical use. 2©008 Elsevier & Formosan Medical Association.