Akademik Veri Yönetim Sistemi
15th International Medicine and Heath Sciences Researches Congress, Ankara, Türkiye, 23 - 24 Mart 2024, ss.213-214
Abstract: Collagen is one of the key components of the extracellular matrix and plays a vital role in
providing structural support and durability in tissue. Valproic acid (VPA) is used as an anticonvulsant
and mood stabilizer in psychiatric and neurological disorders, but is associated with many toxicities. It
is known that VPA causes a decrease in collagen in tissues, but its mechanism has not yet been fully
explained. Chard (Beta vulgaris L. var. cicla) extract has beneficial effects in improving various clinical
and pathological conditions due to the many phytochemical and bioactive compounds it contains. This
study aimed to investigate the effect of chard extract on lung collagen in rats administered VPA.
Sprague-Dawley rats were randomly selected and divided into four groups (n=5 in each): control (C)
group; control+chard (CC) group (100 mg/kg per day, gavage); VPA (V) group (500 mg/kg per day,
intraperitoneal); and VPA+chard (VC) group (at the same doses). The amount of collagen detected using
Sirius Red and Fast Green dyes in lung tissue sections taken from rats sacrificed on the eighth day was
evaluated. The amount of collagen was significantly increased in the CC (p<0.01) and VC (p<0.05)
groups compared to the C and V groups, respectively. There was no significant difference between C and V groups (p>0.05). The therapeutic potential of VPA to reduce collagen synthesis was not seen in
this study; on the contrary, chard extract significantly increased the amount of lung collagen. Therefore,
it was concluded that people at risk of lung fibrosis should limit their consumption of large amounts of
chard and more detailed research on the subject should be conducted