Akademik Veri Yönetim Sistemi
Turk Hijyen ve Deneysel Biyoloji Dergisi, cilt.79, sa.3, ss.419-432, 2022 (Scopus)
© 2022,Turk Hijyen ve Deneysel Biyoloji Dergisi. All Rights Reserved.Objective: Although Coagulase Negative Staphylococci (CoNS) species are among the microbiota members of the skin and mucosal surfaces, they cause infections especially related to medical devices such as catheters. Nowadays, antibiotic resistance is an important public health problem and rational use of antibiotics is of great importance in the treatment of bloodstream infections caused by CoNS species. The aim of this study was to investigate the antibiotic resistance profile and some virulence genes of CoNS isolates that cause catheter related bacteremia. Methods: In our study, 43 CoNS strains isolated from patients hospitalized in Manisa Celal Bayar University Hospital between 2016-2017, were evaluated for their susceptibility to ampicillin, clindamycin, mupirocin, linezolid, tigecycline, tetracycline, cefotaxime, gentamicin, vancomycin, ciprofloxacin, erythromycin and fusidic acid. The presence of clonal relationships and some virulence factors were also investigated. Susceptibility of cefoxitin (methicillin) was determined by disk diffusion and susceptibilities of other antibiotics were determined by microdilution method. AacA-aphD and aphA3 for aminoglycoside resistance, mecA and mecC for methicillin resistance, tetK and tetM for tetracycline resistance, ermA, ermB, ermC, msrA for macrolidelincosamide-streptogramin (MLS) type B resistance, icaA, IS256, nucA and sasX virulence genes were investigated by polymerase chain reaction (PCR). Clonal relationships at species level were examined by Enterobacterial Repetitive Intergenic Consensus (ERIC)-PCR. Results: Considering all species of CoNS examined, the highest and the lowest resistance rates were observed for ampicilin (93 %) and linezolid (2 %), respectively. There wasn’t any resistant CoNS isolate to tigecycline and vancomycin. We found that all the examined CoNS species were generally clustered in different clones. We detected mecA, aacA-aphD, aphA3, ermB, ermC and msrA antibiotic resistance genes as well as icaA, IS256 and sasX virulence genes in the studied strains. Conclusion: The results of our study revealed that the examined CoNS isolates causing bacteremia had acquired resistance to commonly used antibiotics and that these strains harboring various virulence genes. Therefore, those isolates and their infections should be carefully monitored.