PEG-based hydrogel-coated test strip for on-site urea determination
International Journal of Polymeric Materials and Polymeric Biomaterials, vol.68, no.10, pp.597-606, 2019 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 68 Issue: 10
- Publication Date: 2019
- Doi Number: 10.1080/00914037.2018.1482460
- Journal Name: International Journal of Polymeric Materials and Polymeric Biomaterials
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Page Numbers: pp.597-606
- Keywords: Colorimetric biosensor, PEG-based hydrogel, urea test strip, urease
- Istanbul Gelisim University Affiliated: No
Abstract
The urea test strip was prepared using polyethyleneglycol based hydrogel, urease and commercial pH-indicator paper. The swelling behavior of hydrogel indicated the ability of the urea to penetrate into the matrix and allowing the test strip to respond in a short time. The color formed on the strip was distinguished by naked eyes in one minute at room temperature. The linear range for urea was 20–200 mg/dL. The assays have good reproducibility. Relative standard deviation values were found within the range of 3.37–12.95% and 1.68–9.54% for intra- and inter-day assays, respectively.