PEG-based hydrogel-coated test strip for on-site urea determination
International Journal of Polymeric Materials and Polymeric Biomaterials, cilt.68, sa.10, ss.597-606, 2019 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 68 Sayı: 10
- Basım Tarihi: 2019
- Doi Numarası: 10.1080/00914037.2018.1482460
- Dergi Adı: International Journal of Polymeric Materials and Polymeric Biomaterials
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.597-606
- Anahtar Kelimeler: Colorimetric biosensor, PEG-based hydrogel, urea test strip, urease
- İstanbul Gelişim Üniversitesi Adresli: Hayır
Özet
The urea test strip was prepared using polyethyleneglycol based hydrogel, urease and commercial pH-indicator paper. The swelling behavior of hydrogel indicated the ability of the urea to penetrate into the matrix and allowing the test strip to respond in a short time. The color formed on the strip was distinguished by naked eyes in one minute at room temperature. The linear range for urea was 20–200 mg/dL. The assays have good reproducibility. Relative standard deviation values were found within the range of 3.37–12.95% and 1.68–9.54% for intra- and inter-day assays, respectively.